Nanoscale tool allows scientists to study membrane proteins one at a time
In biology, as in construction, it鈥檚 all about having tools that fit the job. Researchers at Rockefeller University have now created a tiny tool, more than 10,000 times smaller than the diameter of a human hair, capable of encasing single membrane proteins from living cells. The new system, which resembles a nanoscale sushi roll, will allow investigators to individually stimulate these key proteins with specific molecules and signals in order to precisely define the biological reactions that result.
The Nanoscale Apolipoprotein Bound Bilayers (NABBs), developed by scientists in Rockefeller鈥檚 Laboratory of Molecular Biology and Biochemistry and reported in the Journal of Molecular Biology, is a versatile device that can likely be adapted to any of the myriad transmembrane receptors that direct cell activity by reacting to molecules outside the cell and activating signals inside the cell.
鈥淭oday it is impossible to know exactly what a single protein on the surface of a cell that has thousands of other proteins is doing. It might be acting on its own or binding to one or more other proteins,鈥� says Thomas Sakmar, Richard M. and Isabel P. Furlaud Professor and head of the laboratory and the study鈥檚 senior investigator. 鈥淲ith this tool, we can control the receptor鈥檚 membrane environment and test all possibilities of interaction with ligands, other receptors or other proteins. It鈥檚 one way to figure out how a complex system works.鈥�
Previously, researchers studied the functions of such proteins by investigating literally millions of them floating together in a soup created when cell membranes are broken apart and solubilized chemically. But this method of studying proteins is problematic, the researchers say: The membrane protein mixtures tend to be inhomogeneous and it is difficult 鈥� partially due to poor stability of the isolated proteins 鈥� to purify them in their active state in order to understand what the receptors are doing individually.
The solution, devised by Sakmar, first author Sourabh Banerjee, a graduate student in the Tri-Institutional Chemical Biology Program, and Thomas Huber, a postdoc, arose as the team searched for a way to exquisitely catalogue the functions of individual G-protein-coupled receptors (GPCRs), a large family of transmembrane proteins that are involved in many diseases and are often the target of medicinal agents. The structure they built was developed using a hard-working human transport particle, the high-density lipoprotein (HDL), as a model system. This flat, circular structure is essentially a complex of phospholipids belted together by apolipoprotein A-I (apo A-I) to carry cholesterol and lipids through blood to the liver.
Assuming that evolutionary forces might have already optimized a biological solution to an engineering problem, Huber suggested using apo A-I from zebrafish. 鈥淏ased on the sequence of zebrafish apo A-I, we thought that it may yield structurally homogeneous discs,鈥� Banerjee says. So in their NABB, zebrafish apo A-I (known as zap1) forms a belt that makes two layers of lipids stick together 鈥� like the seaweed that keeps sticky rice together in sushi.
They then devised a method to trigger rapid self-assembly of these disc-like nanoparticles from mixtures of zap1, lipids and extracted cellular membrane proteins. 鈥淲e have made it fairly straightforward to make these structures and they form in less than an hour,鈥� says Banerjee, who coined the term NABBs.
The team visualized individual antibody fragments bound to the receptors with an electron microscope. And, as a proof of principle, they experimented with rhodopsin, a prototypical GPCR. They found that rhodopsin was remarkably stable in NABBs 鈥� as stable as in its native membranes. They also found that it doesn鈥檛 require a 鈥渄imer,鈥� or union of two rhodopsin receptors, to produce a response 鈥� as many scientists had argued 鈥� but that rhodopsin can be activated in its monomeric form.
鈥淓ach protein is very happy inside its own disc and the beauty is that both sides of these receptors, the part that is inside the cell and the part that is outside, are exposed to whatever you want to test it with,鈥� Sakmar says. 鈥淭hat way we can use it to monitor what happens on both sides of the cell membrane.鈥�
鈥淭his tool can be used for a wide variety of membrane proteins,鈥� Banerjee says. 鈥淲e think it will be important for high-throughput screening for new drugs that can bind to membrane proteins involved in disease.鈥�
Citation: Journal of Molecular Biology online: February 2, 2008
Source: Rockefeller University
