麻豆淫院

(麻豆淫院Org.com) -- At fertilization, a massive release of the metal zinc appears to set the fertilized egg cell on the path to dividing and growing into an embryo, according to the results of animal studies supported by the National Institutes of Health.

The zinc discharge follows the egg cell鈥檚 steady accumulation of zinc atoms in the developmental stages before fertilization. The researchers documented the discharge by bathing the eggs in a solution that gives off light when exposed to zinc. They referred to the zinc discharge and accompanying light flash as zinc sparks.

鈥淭he discovery of egg cells鈥� massive intake and later release of zinc defines a new role for this element in biology,鈥� said Louis DePaolo, chief of the Reproductive Sciences Branch at the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), one of the NIH institutes supporting the study. 鈥淲e anticipate the findings will one day lead to information useful for the treatment of infertility as well as the development of new ways to prevent fertilization from occurring.鈥�

The study鈥檚 authors suggest that zinc acts as a switch, turning off the process of cell division while the egg matures and turning it on again after fertilization.

鈥淭hese findings suggest zinc is essential for developing a healthy egg and, ultimately, a healthy embryo,鈥� said Teresa Woodruff, Ph.D., one of the article鈥檚 senior authors.

The study鈥檚 first author is Alison M. Kim, Ph.D., of Northwestern University, Evanston, Ill. The other authors are Miranda L. Bernhardt, Betty Y. Kong, Richard W. Ahn, Dr. Woodruff and Thomas V. O鈥橦alloran, Ph.D., of Northwestern, and Stefan Vogt, Ph.D., of Argonne National Laboratory, Washington, D.C.

Their findings appear in the July issue of ACS Chemical Biology.

In this study, the researchers observed egg cells from mice and from monkeys. To conduct the study, they devised a microscope that would allow them to view the concentration and distribution of zinc atoms in individual cells. With the aid of the chemical that gives off light when exposed to zinc, the researchers documented the first zinc sparks 20 minutes after fertilization. Most fertilized eggs released two or three rounds of sparks, but the researchers saw as few as one and as many as five within the first two hours after fertilization. The sparks flared every 10 minutes, on average.

Previous research had shown that fertilization triggers cyclical changes in the level of calcium in the egg cell. The researchers noted that the zinc sparks always occurred after a peak in calcium levels inside the cell.

鈥淭he number, timing and intensity of these sparks could tell us something important about the quality of the egg and will be an important area for future research,鈥� said Dr. O鈥橦alloran, the article鈥檚 other senior author. 鈥淚t鈥檚 may also be worth investigating whether the amount of zinc in a woman鈥檚 diet plays a role in fertility.鈥�

Additional experiments helped confirm a role for zinc in the fertilization process. Typically, once the egg is released from the ovary, it must get rid of excess chromosomes in two stages as it prepares to fuse with the sperm. The team鈥檚 earlier research showed that the early accumulation of zinc is essential for properly completing the first stage, Dr. O鈥橦alloran explained. The latest results suggest that zinc may act as a brake in between these stages, as the egg awaits fertilization. If the cell is fertilized, the zinc release appears to lift the brake. The cell discards its excess genetic material and begins to divide.

The researchers also showed that even unfertilized eggs would start to divide if zinc levels were artificially reduced, mimicking release. In addition, when fertilized cells were forced to take on additional zinc, the process was reversed.

鈥淲e have shown that zinc appears to regulate this precisely calibrated, intricate process,鈥� Dr. Woodruff said. 鈥淭he findings give us new insights into what these cells need to grow and mature properly.鈥�