Sorting stem cells
When an embryonic stem cell is in the first stage of its development it has the potential to grow into any type of cell in the body, a state scientists call undifferentiated.
A team of researchers from Scotland has now demonstrated a way to easily distinguish undifferentiated embryonic stem cells from later-stage stem cells whose fate is sealed. The results are published in the American Institute of Âé¶¹ÒùÔºics' (AIP) journal Biomicrofluidics.
The researchers used an electric field to pull stem cells through a fluid in a process called dielectrophoresis. They varied the frequency of the voltage used to generate the electric field and studied how the cells moved, a response that was affected by the cell's electrical properties.
The researchers found that differentiated stem cells could store a significantly greater charge on their outer membranes, a characteristic that might be used to effectively identify and separate them from undifferentiated cells.
The researchers write that the wrinkling, folding, and thinning of a cell's membrane as it differentiates may explain why the later-stage cells can store more charge. The sorting method may prove useful in separating cells for biomedical research or ultimately for treatments of diseases such as Parkinson's.
More information: "Dielectrophoresis based discrimination of human embryonic stem cells from differentiating derivatives" is published in the journal Biomicrofluidics:
Journal information: Biomicrofluidics
Provided by American Institute of Âé¶¹ÒùÔºics