麻豆淫院

Janelia researchers are decoding how neurons carry out computations carefully calibrated to an animal's movement and environment to precisely regulate the release of neuromodulators鈥攃hemicals that fine-tune brain activity and enable us to adapt to new situations.

The new findings could help scientists better understand how the brain enables flexible behavior and provide insight into mood disorders like depression.

Unlike neurotransmitters, which enable fast communication between neurons, neuromodulators regulate groups of neurons over slower timescales. These chemicals adjust how our brain responds to messages and help shape our behavior, mood, and thinking.

In larval zebrafish, the neuromodulator serotonin controls how hard the fish swims as changes in the environment or its own body alter the effectiveness of its effort. from the Ahrens Lab at Janelia found that serotonergic neurons in a brain region called the dorsal raphe nucleus use visual clues to assess the effectiveness of the fish's swimming to figure out how much effort it should exert in the future, releasing serotonin to adjust the fish's swimming vigor.

In appearing in Neuron, a team led by the Ahrens Lab sought to understand how these neurons figured out when and how much serotonin to secrete. While much research has focused on how neuromodulators affect neuronal circuits, less is known about how the neuromodulatory system itself processes information.

As the fish swam in a virtual reality setup, the researchers tracked activity in the raphe using voltage sensors and neurotransmitter imaging tools developed at Janelia.

Zebrafish swim in a staccato pattern鈥攕wimming and coasting鈥攁nd they found that serotonergic neurons in the raphe only integrate information about their perceived motion right after a period of swimming. Ahrens and his team determined that a "gate" allows visual information to enter the raphe: It is closed if the fish hasn't swum, and opens just after it does.

This process enables cells in the raphe to use visual information associated with the fish's swimming effort to adjust the fish's actions while filtering out irrelevant visual signals. Generally, this type of credit assignment鈥攖o correctly associate actions with outcomes鈥攊s at the heart of how a system learns from experience and is an active field of research in neuroscience and machine learning.

Next, the team examined how this gating works. Surprisingly, they found that swimming initially suppresses activity in the serotonergic neurons in the raphe. But, once the fish stops swimming and enters the coasting period, the suppression is removed.

This causes a rebound effect, increasing neuronal activity鈥攎uch like pushing down on a balloon on a table and then releasing it, causing it to spring upwards. During this rebound phase, the gate opens, and visual information can reach the raphe. These visual signals add to the excitation of the neurons and cause the cells to fire in proportion to the visual speed, releasing serotonin that adjusts the fish's swimming vigor.

The new findings provide insight into how neuromodulation works in the raphe鈥攁 brain region also found in human brains鈥攁nd potentially shed light on the processes involved in other neuromodulatory systems in the brain, according to the researchers.