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Electron microscope technique achieves sub-Ångström resolution with lower cost and energy

New technique boosts electron microscope's clarity
Gold on amorphous carbon reconstruction results. Credit: Nature Communications (2025). DOI: 10.1038/s41467-025-64133-3

A team of researchers at the University of Victoria (UVic) have achieved an advance in electron microscopy that will allow scientists to visualize atomic-scale structures with unprecedented clarity using lower-cost and lower-energy microscopes than ever before.

Led by Arthur Blackburn, co-director of UVic's Advanced Microscopy Facility, the team developed a novel imaging technique that allowed them to achieve sub-Ångström resolution using a compact, low-energy scanning (SEM)—a feat previously possible only with a large, high-cost transmission electron microscope (TEM).

The research, published in , opens the door to more accessible microscopy for labs around the world. The new technique allows for high-resolution, atomic-scale images without the previously prohibitive cost, space and personnel requirements.

The breakthrough was made possible by applying a technique called ptychography, which uses overlapping patterns of scattered electrons to build a highly detailed picture of a sample.

Using this technique, the team was able to reach a resolution of just 0.67 Ångström—less than the size of an atom, and 1/10,000 the width of a human hair—using a low-energy beam on a SEM. Previously, achieving sub-Ångström resolution required a high-energy beam and a TEM.

"This work shows that high-resolution imaging doesn't have to rely on expensive, complex equipment. We've demonstrated that a relatively simple SEM, when paired with advanced computational techniques, can achieve a that rivals or even surpasses traditional methods," said Blackburn, Hitachi High-Tech Canada Research Chair in Advanced Electron Microscopy.

"This could be transformative for fields like , nanotechnology and structural biology.

"The advance will most immediately benefit the research and production of 2D materials, which are promising in the development of next-generation electronics. Long term, it could also assist in determining the structure of small proteins, leading to advances in health and disease research."

More information: Arthur M. Blackburn et al, Sub-ångström resolution ptychography in a scanning electron microscope at 20 keV, Nature Communications (2025).

Journal information: Nature Communications

Citation: Electron microscope technique achieves sub-Ångström resolution with lower cost and energy (2025, October 15) retrieved 15 October 2025 from /news/2025-10-electron-microscope-technique-ngstrm-resolution.html
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