麻豆淫院

(麻豆淫院Org.com) -- UConn scientists say the results could complicate disease identification in humans.

A new genomics study by molecular biologists at the University of Connecticut has shown that at least 22 percent of non-human genome databases are contaminated with human DNA. Their results imply that this level of contamination could also exist in records of the human genome, which could produce major problems in identifying human diseases.

Associate professor Rachel O鈥橬eill, graduate student Mark Longo, and associate professor Michael O鈥橬eill of the molecular and cell biology department in the College of Liberal Arts and Sciences published their findings today in an online edition of the journal PLOS One.

Longo says that he had originally been scanning the genome of zebrafish and comparing it with the human genome to find what are called ultraconserved regions, or bits of DNA that are so ancient they are similar among species that are distantly related, like humans and fish.

But, to Longo鈥檚 surprise, he found a region of DNA that was identical to one in humans and couldn鈥檛 be a part of the fish genome. That鈥檚 when he knew that the fish genome database he was using was contaminated.

鈥淐ontamination in these databases could be from people鈥檚 skin or hair, or it could be DNA from other sequence libraries kept in the same facility,鈥� says Longo. 鈥淲e knew we needed to quantify this to see how many of the databases contained human contamination.鈥�

The researchers gathered sequences from all the major global DNA repositories, including the archives at the National Center for Biotechnology Information, the University of California Santa Cruz, the Joint Genome Databases, and the Ensembl genome browser. Any sequencing project funded by federal funds is required to be deposited in one of these archives.

Using a section of DNA that is specific to primates and abundant in the human genome, the researchers identified 454 non-primate genomes out of the 2,027 they sampled as contaminated with human DNA.

Rachel O鈥橬eill says this result led them to reason that if these non-human genome databases were contaminated with human DNA, then it鈥檚 just as likely that many human databases would be contaminated as well. But, she says, the catch is that it鈥檚 virtually impossible to identify a foreign bit of human DNA in a human genome database.

鈥淚n sequencing, you have to put all the pieces of the genome together like a big jigsaw puzzle. The pieces that don鈥檛 fit stand out,鈥� Longo says. 鈥淏ut if you鈥檙e working on a human puzzle, it鈥檚 like working on a three-billion piece puzzle, and it鈥檚 all black.

鈥淚t鈥檚 virtually impossible to find human contamination in human genome databases,鈥� she adds, because they simply don鈥檛 stand out as anything unusual in a human genome. This, she says, could lead to some terrible mistakes.

A portion of the National Center for Biotechnology Information includes a Cancer Genome Atlas: a library documenting mutations that occur in cancer cells. O鈥橬eill says there鈥檚 no room for error in these databases.

鈥淚t would be very upsetting to be told you have a mutation for breast cancer, when in fact you don鈥檛, and it was just a contamination from another sample,鈥� she says.

O鈥橬eill emphasizes that scientists need to exercise extreme caution when performing their sequencing, and that they should validate results through tests in their own laboratories before submitting them to databases. Longo points out that the UConn researchers found contaminations in some sequences that they had produced in their own laboratories, which they then discarded. O鈥橬eill says these practices should be the norm.

鈥淲e鈥檙e compounding this problem in our rush to move forward with genomics,鈥� she says. 鈥淢illions of dollars are invested each year in these sequence databases, but we鈥檙e plowing ahead with less caution than we should. The result is that we might have a harder time recognizing the etiology of something like cancer.鈥�

Longo notes that in his analysis, there was one type of DNA database that showed no contamination at all: that of influenza. Because viruses are so dangerous, great care is taken in their preparation, he says 鈥� much more than is usually taken with a commonplace and harmless genome. This kind of caution should be extended to all sequencing, says O鈥橬eill.

鈥淭he sequencing world has moved in leaps and bounds,鈥� she says. 鈥淚t鈥檚 time for validation to catch up.鈥�