麻豆淫院

The shape of chromosomes is determined by the relative levels of key protein complexes, research conducted by Keishi Shintomi and Tatsuya Hirano of the RIKEN Advanced Science Institute has shown.

As a cell prepares to divide via the process called mitosis, chromatin鈥攖he material in which DNA is packaged鈥攃ondenses to form discrete rod-shaped structures called chromosomes. Each chromosome contains duplicated chromatids鈥攕ister chromatids鈥攖hat are aligned in parallel. After 鈥榤itotic chromosome condensation鈥� is complete, the paired chromatids segregate such that each daughter cell receives one of each pair.

鈥淔or well over a century, biologists have noticed that the shape of condensed chromosomes is highly characteristic, but varies among different organisms or among different developmental stages in a single organism,鈥� explains Hirano. 鈥淲e are interested in understanding how the shape of chromosomes is determined at a molecular level.鈥�

Hirano鈥檚 group previously discovered that mitotic chromosome condensation requires the action of two protein complexes, known as condensins I and II. This group and others have shown that a third protein complex called cohesin is responsible for the pairing of sister chromatids within a chromosome. 

To test exactly how condensins and cohesin may contribute to shaping of chromosomes, Shintomi and Hirano turned to a cell-free system based on extracts prepared from the eggs of the frog Xenopus laevis. 鈥淭he Xenopus system perfectly suited our purposes because it enables us to recapitulate many chromosomal events, including chromosome condensation, in a test tube in a cell-cycle regulated manner (Fig. 1),鈥� says Hirano. 

To achieve their goal, the researchers then had to develop a series of sophisticated experimental protocols to precisely manipulate the levels of condensins I and II and cohesin present in the extracts.

Under the standard condition, chromosomes assembled in this cell-free system tended to be long and thin, which are general characteristics of chromosomes observed in early embryos. Strikingly, however, when the ratio of condensin I to II was reduced, they became shorter and thicker, being reminiscent of chromosomes observed in later stages of development. Further experiments revealed that cohesin works with condensin I and counteracts condensin II to properly place sister chromatids within a chromosome. Thus, their actions can be likened to a molecular 鈥榯ug-of-war鈥�.

鈥淥ur findings demonstrated that chromosome shape is achieved by an exquisite balance between condensin I and II and cohesin,鈥� says Hirano. 鈥淪uch a concept had been suspected for a long time, but has never been demonstrated so beautifully and convincingly until now.鈥�