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Biological clocks: How does the body know that time goes by?

In April of this year, Spanish athlete Beatriz Flamini emerged into the light after a 500-day stay in a cave. Her descent underground is by a long stretch. Flamini says she lost all sense of time on the 65th day. But can she really be sure it was the 65th day? By way of comparison, in 1962 France's Michel Siffre surfaced from the Scarasson chasm in Italy after spending what he thought was 33 days there. In fact, he spent .

The tick of life's clocks

How can isolated human beings keep regular track of time, even when they're disconnected from their surrounding environment? Quite simply, because biological rhythms are at the heart of life, regulating it all the way from the molecular level up to that of the entire body. These include not only our sleep/wake cycles, but also , to name but a few.

And these rhythms have many repercussions, not least in terms of public health. Indeed, a number of diseases are episodic—for example, , while cardiovascular accidents are more frequent in the morning. Another example is shift work, which disconnects people from their environment. It may be associated with an increased risk of cancers in workers, prompting the WHO to label it as a .

Rhythms also impact how we interact with other species. For example, African trypanosomiasis, also called sleeping sickness, is a caused by the parasite Trypanosoma brucei, whose —just like our .

Genes: the great clockmakers

The rotations of the Earth, moon and sun generate environmental cycles that have favored the selection of .

A biological clock is a mechanism internal to organisms that, in the absence of an environmental signal, operates at its own frequency. The regular alternation of day and night has, for example, favored the evolution of the circadian clock (circa, meaning "approximately", and diem, "day").

The , also known as Drosophila, in the 1970s. It is based on feedback loops in the transcription and translation of several genes—gene A promotes the expression of gene B, which in turn inhibits the expression of gene A—creating an oscillation. During the day, light induces the diminution of specific factors of the loop via a photoreceptor called cryptochrome. Interestingly, the key factors in the mechanism essentially only comprise a few genes named . However, the fine-tuning and regulation of the clock is based on a complex molecular and neuronal network that ensures its timing and precision.

There is no single, overarching circadian clock that would organize all of life, as the clock genes vary from species to species. But the principle remains the same: genes whose expression oscillates. Biological rhythms have been described in all the taxa (groups of organisms) studied so far, which comprise cyanobacteria (a type of bacteria that obtain energy via photosynthesis), fungi, plants, and animals, including humans.

In addition, various time givers (zeitgebers) synchronize the organism with its environment: light (the most studied to date), temperature and food, in particular.

An internal clock synchronized by the environment

One very concrete implication of this circadian clock concerns . This is the deviation of an individual's internal rhythm from the time of the time zone they are in.

Environmental signals in general, and light in particular, help to re-synchronize the individual: light perceived at the end of the night moves the clock forward, while light perceived at the beginning of the night delays it. Light perceived during the day has no effect. In humans, light is not perceived directly by the molecular clock, but is captured in the retina and then transmitted via the retino-hypothalamic pathway to a central clock, where it modulates the synthesis of clock proteins. The system is not infinitely scalable, however: it takes the human body approximately one day to adapt to a one-hour time difference.

With Homo sapiens' intrinsic circadian period spanning , it is easier for us to travel west and lengthen our days than to travel east and shorten them. This is also why athletes and researchers who isolate themselves in the depths of the Earth end up being out of sync with time on the surface, and ultimately perceive fewer days than 24-hour solar days.

Other times, other clocks

The circadian clock is not the only clock mechanism that exists in nature. Many biological processes are , such as the migration of a host of birds and insects, the reproduction and hibernation of many animal species and the flowering of plants. This seasonality is generally dictated by several factors, including by what is known as a in the case of many species. The mechanism of this clock has not yet been determined.

The clock mechanisms in marine species are also unknown, partly because of the oceans' . Marine organisms are exposed to the solar cycle of alternating day and night, which is superimposed on a series of lunar cycles, the most prominent of which is the tidal cycle (with a period of 12.4 hours or 24.8 hours). The semi-lunar and lunar cycles (14.8 days/29.5 days), linked to the phases of the moon, also strongly modulate the marine environment, via light and tides. The seasons also affect these ecosystems.

While complex, the temporal structure of marine environments is predictable, and biological rhythms linked to all these cycles have been described in marine species. For example, many corals , laying eggs once a year over a very short period of time. Some marine worms swarm precisely , in the darkest hours of the night, to initiate their reproductive dance before spawning and dying.

Interestingly, in 2020, our team of scientists revealed that biological rhythms are not limited to the coastal environment. We indeed showed , in a mussel living in the hydrothermal vents of the mid-Atlantic ridge. Our work underlines that the temporal coordination in physiology is likely critical, even in the most extreme life environments such as the deep ocean.