麻豆淫院

Cacao, the chocolate tree, is one of the world's most important economic crops, generating hundreds of billions of dollars annually. However, cocoa is affected by a range of pests and diseases, with some estimates putting losses as high as 30% to 40% of global production.

Now, a team led by researchers at Penn State has created a genetic information resource to help plant breeders develop resistant strains of cacao that can be grown sustainably in its native Amazon and elsewhere, such as the tropical latitudes of Central and South America, the Caribbean, Africa and Asia.

In findings June 26 in BMC Plant Biology, the team described the Cacao Gene Atlas, a huge, compiled dataset of replicated transcriptomes the researchers started in 2016.

Transcriptomes are the protein-coding part of the organism's genome that can be analyzed by researchers to determine when and where each gene is turned on or off in cells and tissues. The researchers' long-term goal is to accelerate breeding to develop high-yielding elite varieties of cacao.

The Cacao Gene Atlas, which contains 11.2 million gene expression data points, is a genomics resource freely available to the public, according to team leader Mark Guitinan, J. Franklin Styer Professor of Horticultural Botany and professor of plant molecular biology in Penn State's College of Agricultural Sciences.

Researchers can use the atlas to simulate how knocking out or enhancing a gene may influence the plant, allowing them to test hypotheses without the expense or time of growing the plant.

"In seconds, the atlas can be used to display expression patterns of any cacao gene throughout the lifecycle of cacao鈥攖his will aid in the discovery of genes for important traits and to accelerate breeding for new varieties of cacao far into the future," he said.

"The atlas can be expanded in the future by adding new data as it becomes available. Ultimately, we hope this serves the cacao farmers of the world by helping them combat plant disease and to produce quality cocoa beans for industry."

Cacao is sometimes called an orphan crop because there are not as many genetic resources readily available for the chocolate tree as for crops such as corn, rice or cotton. This research changes that dynamic, according to co-first author Evelyn Kulesza, doctoral degree candidate in plant biology.

"For those other crops, genomic resources already exist for scientists to use for various gene analyses," she said. "We created this resource that allows many other cacao scientists around the world to easily conduct research. We expanded the knowledge base, and it allows other scientists who maybe don't have the monetary support or lab space to conduct experiments to test how different genes behave."

"We extracted RNAs and sequenced transcriptomes from 123 different tissues and stages of development representing major organs and developmental stages of the cacao lifecycle," Thomas said. "In addition, we performed several experimental treatments and time courses to measure gene expression in tissues responding to biotic and abiotic stressors."

To promote wider use of the information, the researchers also made all raw gene-sequencing data, gene-expression mapping matrices, scripts and other relevant specifics used to create the atlas freely available online. The researchers developed a gene-expression browser with graphical user interface to display gene-expression patterns and to provide easy access to raw data and statistical analyses.