Â鶹ÒùÔº

Tiny laboratories that fit in your hand can rapidly identify pathogens using electricity

When you think of electric fields, you likely think of electricity—the stuff that makes modern life possible by powering everything from household appliances to cellphones. Researchers have been studying the principles of electricity . , famous for his kite experiment, demonstrated that lightning was indeed electrical.

Electricity has also enabled major advances in biology. A technique called allows scientists to analyze the molecules of life—DNA and proteins—by separating them by their electrical charge. Electrophoresis is not only commonly taught in high school biology, but it's also a workhorse of many clinical and research laboratories, .

I am a who works with miniaturized electrophoretic systems. Together, my students and I develop portable versions of these devices that rapidly detect pathogens and help researchers fight against them.

What is electrophoresis?

Researchers discovered electrophoresis by applying an electric voltage to clay particles and observing how they migrated through a layer of sand. After further advances during the 20th century, electrophoresis became standard in laboratories.

To understand how electrophoresis works, we first need to explain . These are invisible forces that electrically charged particles, such as protons and electrons, exert on each other. A particle with a positive electrical charge, for example, would be attracted toward a particle with a negative charge. The law of "opposites attract" applies here. Molecules can also have a charge; whether it's more positive or negative depends on the types of atoms that make it up.

In electrophoresis, an electric field is generated between two electrodes connected to a power supply. One electrode has a positive charge and the other has a negative charge. They are positioned on opposite sides of a container filled with water and a little bit of salt, which can conduct electricity.

When charged molecules such as DNA and proteins are present in the water, the electrodes create a force field between them that pushes the charged particles toward the oppositely charged electrode. This process is called .

Researchers like electrophoresis because it is fast and flexible. Electrophoresis can help analyze distinct types of particles, from molecules to microbes. Further, electrophoresis can be carried out with materials such as paper, gels and thin tubes.

In 1972, physicist and his colleagues observed another type of electrophoretic migration called that could separate particles not only by their electrical charge but also by their size and shape.

Electric fields and pathogens

Further advancements in electrophoresis have made it a useful tool to fight pathogens. In particular, the microfluidics revolution made possible the tiny laboratories that allow researchers to rapidly detect pathogens.

In 1999, researchers found that these tiny electrophoresis systems could also by differences in their electrical charge. They placed a mixture of several types of bacteria in a very thin glass capillary that was then exposed to an electric field. Some bacteria exited the device faster than others due to their distinct electrical charges, making it possible to separate the microbes by type. Measuring their migration speeds allowed scientists to identify each species of bacteria present in the sample through a process that took less than 20 minutes.

Microfluidics improved this process even further. Microfluidic devices are small enough to fit in the palm of your hand. Their miniature size allows them to perform analyses much faster than conventional laboratory equipment because particles don't need to travel that far through the device to be analyzed. This means the molecules or pathogens researchers are looking for are more easily detected and less likely to be lost during analysis.

For example, samples analyzed using conventional electrophoresis systems would need to travel through capillary tubes that are about 11 to 31 inches (30 to 80 centimeters) long. These can take 40 to 50 minutes to process and are not portable. In comparison, samples analyzed with migrate through microchannels that are only 0.4 to 2 inches (1 to 5 centimeters) long. This translates to small, portable devices with analysis times of about .

Nonlinear electrophoresis has enabled more powerful devices by allowing researchers to separate and detect pathogens by their size and shape. My lab colleagues and I showed that combining nonlinear electrophoresis with microfluidics can not only but also .

Tiny electrophoresis systems in medicine

Microfluidic electrophoresis has the potential to be useful across industries. Primarily, these small systems can replace conventional analysis methods with .

For example, when , these tiny devices could help researchers quickly tell whether pathogens are dead after treatment. It could also help doctors decide which drug is most appropriate for a patient by quickly distinguishing between normal bacteria and antibiotic-resistant bacteria.

My lab is also working on developing microelectrophoresis systems for purifying bacteriophage viruses that can be used to .

With further development, the power of electric fields and microfluidics can speed up how researchers detect and fight pathogens.